| DNA |
| Plattform |
Amplification Step |
Sequencing Chemistry |
Average Read Length (bases) |
Run Time |
Reads per Run |
Bases per Run |
| Sanger 330XL |
Yes (PCR) |
Chain termination by dideoxynucleotides |
400 to 900 |
20 minutes to 3 hours |
96 |
1,900 to 84,000 |
| Pacific Biosciences/ PacBio RS† |
No (single molecule) |
Real-time single-molecule sequencing reactions |
1,500 (C1 chemistry) |
2 hours |
45,000 |
100,000,000 |
| 454 GS FLX |
Yes (emulsion PCR) |
Pyrosequencing detection of pyrophosphate release with incorporation of
correct complementary base |
700 |
24 hours |
1,000,000 |
700,000,000 |
| Ion Torrent (Personal Genome Machine, 318 Chip) |
Yes (emulsion PCR) |
Detection of pH change by semiconductor technology with incorporation of correct complementary base |
200 |
2 hours |
5,000,000 |
1,000,000,000 |
| SOLiD 4 |
Yes (emulsion PCR) |
Sequencing by ligation using four fluorescently labeled di-base probes |
50 paired end |
14 days |
1,400,000,000 |
120,000,000,000 |
| Illumina/HiSeq 2000 |
Yes (bridge amplification PCR) |
Sequencing by synthesis using basespecific fluorophores and cyclic
reversible-chain termination |
100 paired end |
3 to approximately 10 days |
3,000,000,000 |
600,000,000,000 |
|